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通过认证 [诚信档案]
| Cat. Number |
HM0064
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| Chemical Name |
Super script Ⅱ阴性逆转录酶
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| Appearance |
悬浮液
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| Application Notes |
BR,生化研究
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| Synonym |
Superscript II Reverse Transcriptase
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| Storage condition |
-20℃
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| References |
来源：Purified from E. coli expressing the pol gene of M-MLV (1,2), mutagenized to reduce the RNase H activity 活力定义：One unit of Superscript® II is the amount of enzyme required to incorporate 1 nmole of eoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)12-18 as template•primer (3). Unit Reaction Conditions：50 mM Tris-HC1 (pH 8.3), 40 mM KC1, 6 mM MgC1 2 , 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18 , 0.1 mg/m1 BSA, and enzyme in 50 µl for 10 min. at 37℃ Performance and Quality Testing：SDS-PAGE purity；endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product Enzyme Function：RNA dependent DNA Polymerase Final Product Size：12.3 kb or less Number of Reactions：10 Reactions Optimal Reaction Temperature：42℃ Sensitivity：Medium Template：ssRNA；ssDNA；RNA-DNA Hybrid 产品描述：Superscript® II Reverse Transcriptase (RT) is an improved version of Superscript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations rather than a deletion in the RNase H active center. Like Superscript® RT, Superscript® II RT has reduced RNase H activity. Unlike Superscript® RT, however, the selective mutations within the RNase H domain maintain full polymerase activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives Superscript® II RT superior performance characteristics, including：Greater first-strand cDNA yields；More full-length cDNA synthesis；Full activity at 42℃
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