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References
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dsGreen, an equivalent of SYBR® Green I is a sensitive dsDNA binding dye, which can be used for routine DNA detection in agarose and polyacrylamide gels. A qPCR grade reagent is also available.
Unlike ethidium bromide, dsGreen is highly selective towards double stranded DNA, much less harmful, and offers better sensitivity.
Comparison between ethidium bromide and dsGreen
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Feature
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Ethidium Bromide
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dsGreen
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Fluorescence
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Red (615 nm)
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Green (524 nm)
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Excitation maximum
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302 nm
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454 nm
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Excitation light source
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UV only
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Blue light or UV
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Sensitivity
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2 ng / band (dsDNA)
100 ng / band (RNA)
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0.08 ng / band (dsDNA)
1–2 ng / band (oligonucleotides)
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Health hazard
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High
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Low
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Recommended protocol
DNA Staining in Gels with dsGreen or SYBR Green I
Note: Fluorescence properties of SYBR Green I bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
Spectral properties
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Excitation maximum, nm:
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454
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ε, L⋅mol−1⋅cm−1:
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73000
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Emission maximum, nm:
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524
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Fluorescence quantum yield:
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0.8
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Product citations
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Zietzer, A.; Buschmann, E.E.; Janke, D.; Li, L.; Brix, M.; Meyborg, H.; Stawowy, P.; Jungk, C.; Buschmann, I.; Hillmeister, P. Acute Physical Exercise and Long-Term Individual Shear Rate Therapy Increase Telomerase Activity in Human Peripheral Blood Mononuclear Cells. Acta Physiologica, in press. doi:10.1111/apha.12820
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Diaz-Romero, J.; Kürsener, S.; Kohl, S.; Nesic, D. S100B+A1 CELISA: A Novel Potency Assay and Screening Tool for Redifferentiation Stimuli of Human Articular Chondrocytes. Journal of Cellular Physiology, 2017, 232(6), 1559–1570. doi: 10.1002/jcp.25682
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Richter-Heitmann, T.; Eickhorst, T.; Knauth, S.; Friedrich, M.W.; Schmidt, H. evaluation of Strategies to Separate Root-Associated Microbial Communities: A Crucial Choice in Rhizobiome Research. Frontiers in Microbiology, 2016, 7, 773. doi: 10.3389/fmicb.2016.00773
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Jain, D.; Siede, W. Rad5 Template Switch Pathway of DNA Damage Tolerance Determines Synergism between Cisplatin and NSC109268 in Saccharomyces cerevisiae. PLoS ONE, 2013, 8(10), e77666. doi:10.1371/journal.pone.0077666
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