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References
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上海惠诚生物提供中性TLC分析生化实验用糖鞘脂,糖鞘脂(glycosphingolipid,GSL)是由神经酰胺和非脂的糖基部分寡糖链构成,鞘脂包括中性鞘糖脂(糖基不含唾液酸)和酸性鞘糖脂
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Formula Weight:mixture
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Unit:1 mg/ml, 1ml
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Solvent:chloroform/methanol, 2:1
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Source:natural
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Purity:mixture
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Analytical Methods:TLC
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Natural Source:bovine and porcine
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Solubility:chloroform/methanol, 2:1
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Physical Appearance:liquid
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Storage:-20°C
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Composition:cerebrosides, lactosylceramides, ceramide trihexosides, globosides
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Dry Ice:No
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Application Notes:
This standard contains four neutral glycosphingolipids for the qualitative identification of unknowns. The mixture is prepared from high purity stock material and contains sphingolipids having one, two, three, and four saccharides. Carbohydrate structures on these glycosphingolipids are synthesized by the sequential addition of monosaccharides by glycosyltransferases. Hence, globoside is derived from ceramide trihexoside which is derived from lactosylceramide which is derived from cerebroside. Glycosphingolipids are involved in many neuronal and degenerative diseases and are therefore being closely investigated in many research areas. This qualitative standard is intended for the use in verifying the identity of unknown samples and is not designed for the quantitative analysis.
参考文献References:
1. A. Cremesti and A. Fischl “Current methods for the identification and quantitation of ceramides: An overview” Lipids, Vol. 35:9 pp. 937-945, 2000
2. J. Bielawski et al. “Comprehensive Quantitative Analysis of Bioactive Sphingolipids by High-Performance Liquid Chromatography–Tandem Mass Spectrometry” Lipidomics, Vol. 579 pp. 443-467, 2010
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