氨[快速]检测试剂盒

氨[快速]检测试剂盒 , Ammonia (Rapid) Assay Kit , 货号：K-AMIAR

品牌：Megazyme

货号：K-AMIAR

中文品名：氨[快速]检测试剂盒

品名：Ammonia (Rapid) Assay Kit

规格： 96次(手工) / 960次 (微孔板) / 960次(自动分析仪)

应用：氨[快速]检测试剂盒，用于包括葡萄汁和葡萄酒以及其它食品、饮料样本中氨含量的快速检测。 辅助因子更强的稳定性，溶解后的辅助因子在4°C下可保存超过1年。适合手工、微孔板和自动分析仪分析。

原理：

食品、饮料和其它食材中氨的紫外检测方法

检测方法：分光光度法 @340 nm

反应时间：~ 5分钟

检测限： 0.07 mg/L

应用案例：

葡萄汁、葡萄酒、水果汁、软饮料、奶制品（例如牛奶）、营养食品、酱油、蛋及蛋制品、奶酪、肉、加工肉、海鲜、烘焙产品、化肥、医药、烟草、化妆品、水、凯氏定氮、纸张（和纸板）、水和其他材料 （例如：生物培养、样品等）

方法识别：

基于此原理的检测方法被MEBAK接受认可。

试剂盒组成：

Bottle 1:	缓冲液（36 ml，pH 8），添加α-酮戊二酸和叠氮化钠（0.02% w/v）作为防腐剂
	4°C下稳定超过 2年
Bottle 2:	NADPH
	-20°C稳定超过5年
Bottle 3:	谷氨酸脱氢酶(2.2 mL）
	4°C稳定超过2年
Bottle 4:	氨标准溶液(5 mL, 0.04 mg/mL) 溶于0.02% (w/v)叠氮钠
	4°C稳定超过2年

优点：

参考文献：

Grape and wine analysis: Oenologists to exploit advanced test kits. Charnock, S. C. & McCleary, B. V. (2005). Revue des Enology, 117, 1-5.

Megazyme “advanced” wine test kits general characteristics and validation. Charnock, S. J., McCleary, B. V., Daverede, C. & Gallant, P. (2006). Reveue des Oenologues, 120, 1-5.

Combined intracellular nitrate and NIT2 effects on storage carbohydrate metabolism in Chlamydomonas. Remacle, C., Eppe, G., Coosemans, N., Fernandez, E. & Vigeolas, H. (2014). Journal of Experimental Botany, 65(1), 23-33.

A mutation in GDP-mannose pyrophosphorylase causes conditional hypersensitivity to ammonium, resulting in Arabidopsis root growth inhibition, altered ammonium metabolism, and hormone homeostasis. Barth, C., Gouzd, Z. A., Steele, H. P. & Imperio, R. M. (2010). Journal of Experimental Botany, 61(2), 379-394.

Proteomic phenotyping of Novosphingobium nitrogenifigens reveals a robust capacity for simultaneous nitrogen fixation, polyhydroxyalkanoate production, and resistance to reactive oxygen species. Smit, A. M., Strabala, T. J., Peng, L., Rawson, P., Lloyd-Jones, G. & Jordan, T. W. (2012). Applied and Environmental Microbiology, 78(14), 4802-4815.

No effects of gluten in patients with self-reported non-celiac gluten sensitivity after dietary reduction of fermentable, poorly absorbed, short-chain carbohydrates. Biesiekierski, J. R., Peters, S. L., Newnham, E. D., Rosella, O., Muir, J. G. & Gibson, P. R. (2013). Gastroenterology, 145(2), 320-328.

The interaction between high ammonia diet and bile duct ligation in developing rats: assessment by spatial memory and asymmetric dimethylarginine. Huang, L. T., Chen, C. C., Sheen, J. M., Chen, Y. J., Hsieh, C. S. & Tain, Y. L. (2010). International Journal of Developmental Neuroscience, 28(2), 169-174.

Dietary protein excess during neonatal life alters colonic Microbiota and mucosal response to inflammatory mediators later in life in female pigs. Boudry, G., Jamin, A., Chatelais, L., Gras-Le Guen, C., Michel, C. & Le Huërou-Luron, I. (2013). The Journal of Nutrition, 143(8), 1225-1232.

Effects of enzymatic modification of wheat protein on the formation of pyrazines and other volatile components in the Maillard reaction. Lee, S. E., Chung, H. & Kim, Y. S. (2012). Food Chemistry, 131(4), 1248-1254.

使用手册：

K-AMIAR使用手册

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