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西宝生物科技（上海）股份有限公司
地址：上海市张江高科技园区毕昇路299弄11号502室 邮编：201204
电话：021-50272978
西宝市场部 手机：13917439331 QQ：2852385819
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R&D,Larodan
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Anti Iba1, Rabbit
019-19741(For Immunocytochemistry) & 016-20001 (for Western Blotting)
Iba1: ionized calcium binding adapter molecule 1 小胶质细胞/巨噬细胞特异性蛋白抗体
钙离子是已知的所有细胞，包括中枢神经系统（CNS）细胞中最重要的信号调解人之一。钙离子通过与各种钙结合蛋白结合发挥信号活动，其中许多钙结合蛋白被划分成一个大的蛋白家族，EF手性蛋白家族。Iba1是一个17 kDa的EF手性蛋白，在巨噬细胞和小胶质细胞中表达，并在这些细胞的活化过程中表达升高。
本抗体以合成的的Iba1羧基末端序列为抗原，该序列是人，大鼠和小鼠Iba1蛋白质保守序列。本抗体（免疫组化用）特异性与小胶质细胞/巨噬细胞反应，免疫双重染色与单克隆抗体结合，可与GFAP单克隆抗体（(胶质纤维酸性蛋白，Glial Fibrillary Acidic Protein）联合使用对脑组织和细胞培养物进行双重染色。
特点
S特异性检测小胶质细胞与/巨噬细胞，不与神经细胞与星形胶质细胞发生交叉反应。可与人，大鼠，小鼠的Iba1反应
|
货号 |
品名 |
包装 |
用途 |
工作浓度 |
|
019-19741 |
Anti Iba1 polyclonal antibody, Rabbit, for Immunocytochemistry |
50ug(100ul) |
IH |
1-2ug/ml |
|
016-20001 |
Anti Iba1 polyclonal antibody, Rabbit, for Western Blotting |
50ug(100ul) |
WB |
0.5-1ug/ml |
石蜡切片应用方法：
1. 固定
2. 用0.01M PBS缓冲液清洗二次
3. 使用含有0.3% 过氧化氢的甲醇孵育30分钟
4. 用0.01M PBS缓冲液清洗三次
5. 使用封闭液（含有1.5%的山羊血清和1%BSA的0.001M PBS缓冲液）室温孵育2小时
6. 使用含有Anti Iba1 polyclonal antibody的封闭液4℃孵育过夜
7. 用0.01M PBS缓冲液清洗三次
8. 使用含有生物素标记的抗兔IgG（1：200）的封闭液室温孵育1小时
9. 用0.01M PBS缓冲液清洗三次
10. 使用Elite ABC试剂（含有试剂A1：50，试剂B1：50的0.01M PBS溶液）
11. 用0.01M PBS缓冲液清洗三次
12. 过氧化物酶溶液（含有0.01% 过氧化氢和0.05% DAB的0.05M Tris溶液）
西宝市场部 手机：13917439331 QQ：2852385819
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电话：021-50272978 13917439331 QQ：2852385819
