     |
密理博Amicon Ultra-0.5 mL超滤离心管
Millipore Amicon Ultra 0.5ml超滤离心管
Amicon Ultra-0.5 mL Centrifugal Filters for DNA and Protein Purification and Concentration
With their vertical membrane design, Amicon Ultra 0.5 mL filters deliver great performance and lower spin times.
|
货号 |
描述 |
NMWL (KDa) |
包装 |
|---|---|---|---|
|
UFC500308 |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-3 membrane |
3 |
8 |
|
UFC500324 |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-3 membrane |
3 |
24 |
|
UFC500396 |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-3 membrane |
3 |
96 |
|
UFC5003BK |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-3 membrane |
3 |
500 |
|
UFC501008 |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-10 membrane |
10 |
8 |
|
UFC501024 |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-10 membrane |
10 |
24 |
|
UFC501096 |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-10 membrane |
10 |
96 |
|
UFC5010BK |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-10 membrane |
10 |
500 |
|
UFC503008 |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-30 membrane |
30 |
8 |
|
UFC503024 |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-30 membrane |
30 |
24 |
|
UFC503096 |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-30 membrane |
30 |
96 |
|
UFC5030BK |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-30 membrane |
30 |
500 |
|
UFC505008 |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-50 membrane |
50 |
8 |
|
UFC505024 |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-50 membrane |
50 |
24 |
|
UFC505096 |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-50 membrane |
50 |
96 |
|
UFC5050BK |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-50 membrane |
50 |
500 |
|
UFC510008 |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-100 membrane |
100 |
8 |
|
UFC510024 |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-100 membrane |
100 |
24 |
|
UFC510096 |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-100 membrane |
100 |
96 |
|
UFC5100BK |
Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-100 membrane |
100 |
500 |
概述
Applications
Material Supplied
The Amicon Ultra-0.5 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample.
Performance – DNA Concentration
The Amicon Ultra-0.5 device provides the best balance between recovery and spin time for double-stranded DNA for base pairs ranging from 137 to 1159. To achieve maximum PCR product recovery and primer removal with primers greater than 20 bases, one or two additional spins with Tris-EDTA (TE) buffer are recommended.
| Typical Recovery of Nucleotides from Amicon Ultra-0.5 30K Devices | ||||
| PCR Product (base pairs) | PCR Primer (bases) | PCR Recovery (%) | PCR Primer Removal (%) | TE Washes (number) |
| 137 | 10 | ≥95 | ≥90 | 0 |
| 20 | ≥90 | ≥85 | 1 | |
| 48 | ≥90 | ≥75 | 2 | |
| 301 | 10 | ≥90 | ≥90 | 0 |
| 20 | ≥85 | ≥90 | 1 | |
| 48 | ≥90 | ≥80 | 2 | |
| 648 | 10 | ≥95 | ≥90 | 0 |
| 20 | ≥90 | ≥90 | 1 | |
| 48 | ≥95 | ≥90 | 2 | |
| 1159 | 10 | ≥90 | ≥90 | 0 |
| 20 | ≥90 | ≥95 | 1 | |
| 48 | ≥95 | ≥95 | 2 | |
Spin conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume, 20-30 µL final volume, 10 minute spin, n=12. |
||||
Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume. Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=8.
| Concentrate Volume / Concentration Factor | ||||||||||
| 3K device | 10K device | 30K device | 50K device | 100K device | ||||||
| Spin time (min) | Conc. Volume (µL) | Conc. Factor (x) | Conc. Volume (µL) | Conc. Factor (x) | Conc. Volume (µL) | Conc. Factor (x) | Conc. Volume (µL) | Conc. Factor (x) | Conc. Volume (µL) | Conc. Factor (x) |
| 5 | 215 | 2 | 66 | 7 | 42 | 12 | 28 | 18 | 58 | 9 |
| 10 | 114 | 4 | 35 | 14 | 23 | 22 | 20 | 25 | 19 | 26 |
| 15 | 80 | 6 | 22 | 22 | 19 | 27 | 17 | 30 | 15 | 33 |
| 20 | 62 | 8 | 20 | 24 | 17 | 30 | 15 | 33 | 13 | 36 |
| 30 | 48 | 10 | 15 | 31 | 15 | 32 | 15 | 36 | 11 | 41 |
| Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume. Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=12. Shaded volumes were used for the calculation of protein recovery in the Typical Concentrate Recovery table. | ||||||||||
| Marker/Concentration | Molecular Weight | Device NMWL | % Retention | Spin Time (min) |
| α-Chymotrypsinogen (1 mg/mL) | 25,000 | 3K | > 95 | 30 |
| Cytochrome c (0.25 mg/mL) | 12,400 | > 95 | 30 | |
| Vitamin B-12 (0.2 mg/mL) | 1,350 | < 42 | 30 | |
| α-Chymotrypsinogen (1 mg/mL) | 25,000 | 10K | > 95 | 15 |
| Cytochrome c (0.25 mg/mL) | 12,400 | > 95 | 15 | |
| Vitamin B-12 (0.2 mg/mL) | 1,350 | < 42 | 15 | |
| BSA (1 mg/mL) | 67,000 | 30K | > 95 | 10 |
| Ovalbumin (1 mg/mL) | 45,000 | > 95 | 10 | |
| Cytrochorme c (0.25 mg/mL) | 12,400 | < 35 | 10 | |
| BSA (1 mg/mL) | 67,000 | 50K | > 95 | 10 |
| Ovalbumin (1 mg/mL) | 45,000 | ~ 40 | 10 | |
| Cytrochorme c (0.25 mg/mL) | 12,400 | < 20 | 10 | |
| Thyroglobulin (0.5 mg/mL) | 677,000 | 100K | > 95 | 10 |
| IgG (1 mg/mL) | 156,000 | > 95 | 10 | |
| Ovalbumin (1 mg/mL) | 45,000 | < 30 | 10 | |
| Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume, n=12. | ||||
| Marker/Concentration | Molecular Weight | Device NMWL | Spin Time (min) | Concentrate Volume (µL) | Concentration Factor (x) | Concentrate Recovery (%) |
| Cytochrome c (0.25 mg/mL) | 12,400 | 3K | 30 | 48 | 10 | 98 |
| Cytochrome c (0.25 mg/mL) | 12,400 | 10K | 15 | 22 | 22 | 93 |
| BSA (1 mg/mL) | 67,000 | 30K | 10 | 23 | 22 | 97 |
| BSA (1 mg/mL) | 67,000 | 50K | 10 | 20 | 25 | 92 |
